The Shoot
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The Shoot is a 2010 rail shooter video game for the PlayStation 3, which uses the PlayStation Move controllers.[2] It is developed by Cohort Studios and published by Sony Computer Entertainment for release in North America on 19 October 2010,[1] Australia on 28 October 2010, and Europe on 29 October 2010. It was officially unveiled at the 2010 Game Developers Conference in San Francisco.[2]
Players will traverse through 5 unique Hollywood-style levels such as an Alien Cyborg invasion, a western cowboy-style town as well as horror themes. The game is a Light gun shooter which utilizes the PlayStation Move for an arcade-style sci-fi Shoot 'em up game. The game is mainly a Rail shooter, however there are also other moves using Move; for instance players have to tilt side to side to dodge oncoming missiles or get a better angle on their shot (the latter usually utilised when enemies hide behind innocent human beings), and lower their gun which hiding behind cover or in preparation for a "quick-draw" duel.[4]
Kotaku praised the game's quick and easy take on the shooter genre along with the game's visuals and clever enemy design. Its only criticism was that sometimes controls can feel sluggish.[18] Ars Technica also queried an initial controller lag, but stated that the player soon gets used to it. It also went on to praise the game's price and replay value.[19]
The function of the SHOOT MERISTEMLESS (STM) gene in shoot and floral meristems throughout Arabidopsis development has been analyzed. The results show that STM plays a major role in maintaining shoot and floral meristems. In an allelic series of stm mutants the shoot meristem was either reduced or completely absent in mature embryos and mutant seedling cotyledons showed partial fusion, indicating that the STM gene affects embryonic shoot meristem development and spacing of cotyledons. Postembryonically, stm mutants initiated adventitious shoot development at a position corresponding to the shoot meristem in wild-type. Repetitively initiated defective mutant shoot and floral meristems were consumed during primordia formation and typically terminated prematurely in fused ectopic primordia, indicating that STM is required for continuous shoot and floral meristem function. Analogous defects were observed in stm embryonic and postembryonic development suggesting that similar mechanisms are employed in embryonic and postembryonic organ primordia initiation. Allelic combination suggest different thresholds for STM requirement during plant development. STM requirement could not be bypassed by standard growth factor regimes or by shoot regeneration from calli. The results suggest that STM functions by preventing incorporation of cells in the meristem center into differentiating organ primordia and that this role can completely account for all defects observed in stm mutants. Mutations in the WUSCHEL (WUS) and ZWILLE (ZLL) genes result in defective organization and premature termination of shoot meristems. Genetic interactions between STM, WUS and ZLL were analyzed and the results indicate that STM acts upstream of WUS and ZLL. Therefore, while STM appears to function in keeping central meristem cells undifferentiated, WUS and ZLL seem to be subsequently required for proper function of these cells.
The maintenance of the stem cell niche in the shoot apical meristem, the structure that generates all of the aerial organs of the plant, relies on a canonical feedback loop between WUSCHEL (WUS) and CLAVATA3 (CLV3). WUS is a homeodomain transcription factor expressed in the organizing centre that moves to the central zone to promote stem cell fate. CLV3 is a peptide whose expression is induced by WUS in the central zone and that can move back to the organizing centre to inhibit WUS expression. Within the past 20 years since the initial formulation of the CLV-WUS feedback loop, the mechanisms of stem cell maintenance have been intensively studied and the function of WUS has been redefin
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